Micron particle sorting has an important role in biomedicine, renewable energy, and micro-manufacturing. In this study, a fishbone-shaped micron particle sorting device was designed based on the dielectrophoretic manipulation of micron particles technique. Firstly, the simulation model of multi-physics field coupling is established using experimental data, and the results are calculated and analyzed using the method of finite element analysis. Finally, according to the simulation results, the factors affecting particle manipulation are particle dimensions, number of electrodes, voltage magnitude, and sheath flow rate. Studies have shown that when the sorting particle diameter is different, increasing the sheath flow speed, voltage strength, and the number of electrodes can improve the sorting effect. By modifying the factors affecting the dielectrophoretic manipulation of micron particle sorting, it can make the present design applicable to the sorting of different micron-size particles and provide a significant reference value for the subsequent particle sorting method.