Realizing the sustainable development of agriculture, culture and tourism industry is an important part of promoting comprehensive rural revitalization of China. Based on the investigation of Jiangxi Province, this paper analyzes the development status of agricultural, cultural and tourism industry and the main difficulties of sustainable development. It is considered that China has initially established an attractive and competitive agricultural, cultural and tourism industry system. However, the lack of characteristic development, insufficient industrial integration and brand marketing system have become the main shortcomings restricting the sustainable development of agriculture, culture and tourism industry. Facing the normalization of COVID-19 and huge market potential, the industry needs to closely follow the development goal of rural comprehensive revitalization, and take “specialization”, “integration” and “digitalization” as the path to truly achieve sustainable and rapid development.