In order to implement the concept of green highway and advocate the construction ideas of ecological harmony, resource conservation, service improvement and sustainable development, in the design process of Wujiu expressway, through the investigation and analysis of natural plants in the project area, the plant species in the project area are sorted out, and the shortcomings of the application of existing highway plant resources in the sustainable development of the project area are analyzed. According to the characteristics of Wujiu expressway, the utilization principle of plant materials for the project is determined. In order to meet the needs of slope ecological protection and sustainable development of greening landscape, the selection of plant resources is explored and studied. Starting from the principles of ecological adaptability, species diversity, ornamental value, and economic applicability, a vegetation survey was carried out. By comparing the three-plant slope protection combination schemes, scheme 1 was selected, namely: perennial rye 30% + bermudagrass 10% +Alfalfa 10%+Huzhizi 30%+Amorpha 20% is the best combination of slope protection plants. In addition, olive fruit, as a local economic crop in Wudu, can also be used as road service area, intercommunication area, and waste slag field. High-quality green plant species.