This study focuses on evaluating the effectiveness of AI-assisted AI-based instructional practices for teaching Python programming to business majors. The study analyzed the distribution of students’ final grades by comparing the class that used the traditional teaching model in the 1st semester of the 2022–2023 academic year with the class that introduced the AI-assisted teaching model in the 1st semester of the 2023–2024 academic year. The results showed that the classes with the AI-assisted teaching model showed a significant improvement in the students’ performance, with a significant increase in the percentage of high-performing bands and a significant decrease in the percentage of low-performing bands. Further, an independent samples t-test was conducted on the grades of the two classes using SPSS 26 software, and it was found that there was a significant difference between the two in terms of average grades, and this difference was highly statistically significant. This study demonstrates that the AI-assisted teaching model based on artificial intelligence has significant advantages in improving the teaching quality and student achievement in Python Programming course for business majors.