The harsh climate and environmental hazards contribute to the structural damage of steel bridges. Substantial dynamic loading from heavy trucks can worsen existing cracks. This paper investigates the dynamic behavior of a steel box girder bridge, the Szapáry bridge, with a fractured girder subjected to moving truck loads. Initially, a finite element model simulates the seven-span continuous bridge behavior during static load testing. The model also accurately simulated the dynamic load tests performed. A series of hypothetical damage (fractured girder) and dynamic loading scenarios reveal the effects of truck positions on the damaged bridge’s dynamic response. Dynamic displacement induced due to traffic loading helps evaluate a bridge’s structural health. Results of the parametric analysis highlight that several factors, including truck velocity and position, bridge span length, and truck lateral spacing, significantly affect the dynamic vibration of the fractured bridge. The results offer insight into the effectiveness of dynamic response analysis for conditioned-based maintenance and damage detection.