The concept of partnership between the public and private sectors is considered a new concept in Palestine, as it is a country under Israeli occupation, and previous studies have not focused on examining the impact of knowledge management in achieving the success of partnership projects between the public and private sectors. This study aims to identify the role of organizational culture (OC). in the relationship between knowledge management (KM) and the success of public-private partnership projects (SPPPs) in the construction sector in Palestine. 150 specialists were contacted from different public and private sectors (government, private, and academic employees who have experience in implementing partnership projects), and 130 questionnaires were considered usable. The Smart Pls-4 program was used to analyze the data, and the study concluded that knowledge management has a significant impact on the completion of partnership projects between the public and private sectors. Moreover, knowledge management has an important relationship with the organizational culture of organizations, and the results also showed that OC acts as a mediating influence in the relationship between knowledge management and SPPPs.