This work aims at detecting in which regions the objects in the image are by using information about the intensity of valleys, which appear to surround objects in images where the source of light is in the line of direction than the camera. We present our depth of valleys accumulation method, which consists of two stages: first, the definition of the depth of valleys image which combines the output of a ridges and valleys detector with the morphological gradient to measure how deep is a point inside a valley and second, an algorithm that denotes points of the image as interior to objects those which are inside complete or incomplete boundaries in the depth of valleys image. To evaluate the performance of our method we have tested it on several application domains. Our results on object region identification are promising, specially in the field of polyp detection in colonoscopy videos, and we also show its applicability in different areas.