The purpose of the 2023, three-day 3rd International Conference on Biomedicine and Bioinformatics Engineering (ICBBE 2023), held in hybrid form from 16–18 June 2023 in Nanjing, China, was to create a timely forum for multi-disciplinary discussion related to recent developments on biomedicine and bioinformatics engineering.

As usual, the program of the meeting consisted mainly of keynote speeches and oral presentations, with the former including speeches delivered by five eminent professors from different countries and regions. The keynote speeches covered: i) Molecular techniques reveal more secrets of fermented foods; ii) Targeted pseudouridylation: A novel approach to suppressing nonsense mutations in disease genes; iii) Graph convolutional residual neural networks for the screening of multi-target anticancer compounds; iv) Development of a novel carbon-based coating for biomedical implants; and v) Nucleic acid thermodynamics and its applications in tumor screening. Among these, the critical vision of the first report provided answers to many questions with regard to understanding the role of molecular techniques in discovering the secrets of fermented foods, such as how to evaluate traditional fermented foods.

In the framework of experimental and theoretical approaches, the conference attracted about 120 delegates from all over the world, and addressed a number of highly relevant aspects of biomedicine and bioinformatics engineering. Covering biomedical signal processing and medical information, bioinformatics and computational biology, medical imaging technology and its application, molecular biology, chemistry, pharmacology and toxicology, among other topics, the selected contributions in this special issue provide guidance for future interdisciplinary developments, and emphasize the multi-scale aspects of biomedicine and bioinformatics.

We received various manuscripts for the invited papers and contributions presented at the conference. All of these have been peer reviewed and are collected in this volume. We would like to thank all the anonymous colleagues who acted as referees to assess the suitability of the various articles for publication and inclusion in the series Studies in Health Technology and Informatics from IOS Press. We are confident that the high quality of both the invited and contributed papers contained in these proceedings will be appreciated by the biomedical community.

We would like to express our thanks to all the authors for their time and genuine efforts, and to the reviewers for their fruitful comments during the preparation of this volume. We also acknowledge the support provided in various ways by Henan University and University of Rochester.

The Committee of ICBBE 2023