The ecological niche of an enterprise is composed of ecological niche factors, which affect the long-term development of the enterprise.This paper aims to identify the factors that influence the ecological niche of manufacturing enterprises in the service ecosystem, and explores the constitutive dimensions of ecological niche factors of manufacturing enterprises in the context of digital servitization through the research method of rooting analysis. Four types of ecological niche factors are identified and divided into internal and external dimensions according to whether the ecological niche factors have a direct impact on the business activities of the enterprise. The development history of the digital service business and the current status of its ecological niche are analyzed in the context of the case company, and the future changes of the ecological niche are predicted by fitting the numerical curves of the ecological niche factors for ten years. The study enriches the existing research results of ecological niche theory and provides a measurement basis for the evaluation of ecological niche factors of manufacturing enterprises in the transformation of digital services.