This paper mainly introduces a set of high-speed railway earthquake early warning test system based on LabVIEW, which serves for the indoor prototype test of high-speed railway earthquake early-warning system, which is used to simulate wave generation instead of seismic sensors. From the perspective of indoor prototype simulation, a high-speed railway earthquake early warning (EEW) test system based on virtual instrument (VI) technology is developed. Through the test system, seismic wave and non-seismic interference wave are simulated, generated and sent, so as to carry out test technology research on the current high-speed railway EEW system in China. Based on the virtual instrument development platform LabVIEW, the high-speed railway earthquake warning test system is developed. The function of the test system is verified by an example, and the consistency between the original waveform and the acquisition waveform is analyzed. The results show that the amplitude accuracy of the test system is 0.005 ± 0.002 gal and the acceleration peak time is no difference, that is, the high-speed railway EEW test system based on VI technology is accurate and reliable.