Computer science and technology have gone deep into many fields, including medical care and public health. The network teaching platform, online courses and formative evaluation developed based on computer science and technology have been widely used in the teaching process of public health, and have played a key role in training qualified public health talents. Case-based learning is a teaching method that takes students as the center and cases as the basis, in which students are guided to find, analyze and solve problems by presenting case situations, and closely combining theory with practice, so that they can understand theories, form their own views and improve their abilities. In this study, the necessity and importance of case-based learning, the basic strategy for the construction of case database, and the design of typical cases of food toxicology were investigated in combination with the training objectives of public health major and the basic situation of food toxicology course in our university. At the same time, a case-based learning was applied in the course of food toxicology, and it was found that this teaching mode could effectively improve the students’ academic performance and teaching satisfaction, and should be of great help to students’ ability to obtain information, learn independently and evaluate information, worth popularizing.