Severe adolescent idiopathic scoliosis (AIS) requires surgery to halt curve progression. Accurate insertion of pedicle screws is important. This study reports a newly developed 3D ultrasound (3DUS) to localize pedicles intraoperatively and register a pre-op 3D vertebral model to the surface to be displayed for navigation. The objective was to determine speed of the custom 3DUS navigator and accuracy of pedicle probe placement. The developed 3DUS navigator integrated an ultrasound scanner with motion capture cameras. Two adolescent 3D printed spine models T2-T8 and T7-T11 were modified to include pedicle holes with known trajectory and be mounted on a high precision LEGO pegboard in a water bath for imaging. Calibration of the motion cameras and the 3DUS were conducted prior to the study. A total of 27 scans from T3 to T11 vertebrae with 3 individual scans were performed to validate the repeatability. Three accuracy tests that varied vertebral a) orientation, b) position and c) a combination of location and orientation were completed. Based on all experiments, the acquisition-to-display time was 18.9±3.1s. The repeatability of the trajectory error and positional error were 0.5±0.2° and 0.3±0.1mm, respectively. The a) center orientation, b) position and c) orientation/position on trajectory and positional error were for a) 1.4±0.9° and 0.5±0.4mm, b) 1.4±0.8° and 0.3±0.3mm and c) 2.0±0.8° and 0.5±0.5mm, respectively. These results demonstrated that a high precision real-time 3DUS navigator for screw placement in scoliosis surgery is feasible. The next step will study the effect of surrounding soft tissues on navigation accuracy.