E-commerce is an emerging industry. It has brought new opportunities for global economic development. The update of e-commerce technology and the improvement of e-commerce profit models promote the profitability of e-commerce enterprises. Nowadays too much emphasis was placed on theoretical teaching in the e-commerce courses. The courses lack case analysis for learners to better understand the actual situation of e-commerce. This study analyzed the profit model of existing e-commerce companies, and used case analysis methods to find the profit dilemma and effective breakthrough methods for the profitability of two typical B2C e-commerce companies. With disassembling specific e-commerce cases, this study completed the conversion of e-commerce courses from theoretical teaching to case teaching.