This cross-sectional research aimed to explore the associated factors with nursing practice for patient safety of nurses in Kalasin Hospital, Kalasin Province, Thailand. The 245 samples were randomized which using created questionnaires which was applied to collect their opinion. The results showed that level of nursing practice for patient safety was moderate level (average = 3.55, S.D. = 0.89) in addition to their experience in patient care, knowledge patient safety and safety culture skills was a significant relationship to practice of nursing professional skills with statistical significance (r = 0.625, p-value = 0.001). It was revealed that supporting system and resources sufficient for the top administrative level in hospital were significantly related to nursing practice and patient safety. Policy direction of the hospitals is becoming aware of the importance of transforming organizational culture in order to improve patient safety and nursing practice toward to safety culture as quality awareness in the organization.