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Purpose:
The aim of this study is to investigate the osteogenic induction of the cells in a transcriptional level.
Methods:
The dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) were isolated from human dental pulp and periodontal ligaments, respectively. The cells were identified by assessing the stem cell markers, and characterized by differentiation multipotency. During the osteogenic induction, we detected the gene expression of DSPP, RUNX2, HOSTRIX, BMP-2 and OPN on Day 3, 5, 7, 10 and 14 by real time PCR. Furthermore, the in vitro mineralization of those cells was examined by semi-quantitative determination.
Results:
DPSCs and PDLSCs expressed stem cell markers and were able to differentiate into multiple cell types. The gene expression of ALP, BMP-2, DSPP, OSTRIX OPN, and RUNX2 revealed a distinguished trend during osteogenesis. For the qual- itative study, semi-quantitative determination of mineralization showed that DPSCs maintain more powerful differ- entiation ability than PDLSCs under the same conditions.
Conclusions:
DPSCs are probably a more suitable candidate for tissue engineering than PDLSCs. The 7th day of osteogenesis differentiation is an appropriate time point to investigate the microenvironment involved differences between DPSCs and PDLSCs.