With the increasing demands of professional rehabilitation training for home caregivers, to develop a mode of training with widespread applicability has been emphasized. In order to improve life quality of the impaired elderly, we developed an online training system for the home caregivers to learn knowledge and skills for caring the elderly. We also assessed the acceptance and acceptance factors of the online training system. The results showed that our training system was well accepted by the participants. The perceived usefulness and perceived ease of use were the dominant factors influencing the acceptance of the online course. Therefore, quality management and easy to use, rapid to capture the system are essential for the users to accept the training courses or health information better. An online training system with professional and standard protocols may be popular and well accepted for home caregivers.