Men have always tried to improve their own lifestyle taking advantage of and adapting natural resources to their needs. This happened also with living organisms. Starting from farming and breeding, developments brought about the ability of knowingly modifying the organisms' DNA that contains the instructions to grow and develop every living organism, arising genetic engineer, biotechnology and synthetic biology. Synthetic biology, in particular, is a multidisciplinary area that allows the creation of artificial living organisms starting from synthetically produced DNA material. This ability could be used for good or malicious purposes, rising dual use issues. This scenario has been complicated by the DoItYourself-biology (DIY-Bio) movement's birth, because this is spreading the use of biotechnology beyond the traditional academic and industrial institutions and into the lay public, increasing concerns about biosafety, biosecurity and ethics, even though it has been shown that working with living organisms, especially to create a biothreat, is not that effortless. The continuous improvements of synthetic biology and DIY-bio should be strictly monitored and regulated in order to guarantee a safe and secure world.