A method for image identification based on the pupil size analysis during human-computer interaction will be presented. The data was gathered during the experiment on 3D interactive representation of fairly large picture collections which facilitates browsing through unstructured sets of icons or pictures. The data was gathered using ASL gaze-tracking device. A group of 18 users took part in the experiment. The obtained gaze tracking results have shown that recorded pupil size patterns may indicate the fact of the presented image identification.