The theme of this paper is to analyze the effect of the eye-mask soothing aromatherapy treatment by using the grey model theory. The eye mask agent is made of stress relief floral water, and we lay it over two eyes to relax stress of subjects. Although traditional alterative medicine describes that the floral water can be transported through the blood circulation from the skin to the various organs, and finally reaches the central nervous system, it is lacked scientific verification. Thus, this paper measures the heart rate variability of subjects and finds the relation between the stress relief and eye-mask aromatherapy. The grey GM(0,N) model theory is applied to find the activity relationship of the pressure index to influence factors: heart rate, standard deviation of all normal to normal intervals, total power, very low frequency power, low-frequency power, and high-frequency power. Based on the grey analysis, the action of the eye-mask aromatherapy is constructed with more scientific study than previous works. The results can be a reference to aromatherapy alterative medicine.