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Bone scaffold with fully interconnected pores whose sizes are gradient structured can be prepared by 3-dimensional (3D) printing technology, and has presented important value in tissue engineering. It is known that, in the direction of bone thickness, the composition, structure and performance are continuously varied, and the number and species of related cells distributed in different regions are different. Based on biological 3D printing platform constructed adopting rotary pneumatic multi-nozzle structure and poly-L-lysine (PL) modified matrix materials, and in order to better simulate the spatial morphology of bone tissue and its function and make seed cells rapidly and accurately migrate to the specific region, a novel approach for preparing composite physical/chemical gradient bone scaffold was proposed. Dividing the scaffold into three different regions, the structure property and mechanical performance of different regions were detailedly analyzed. In addition, using adipose derived stem cells (ASCs) as seed cells, their initial adhesion on different regions and corresponding viability analysis were also conducted, respectively. The experiment results show that gradient material can make the cell migrate toward and attatch on specific location and optimizing its physical structure can improve its mechanical properties. Thus the composite gradient scaffold prepared through this approach presents great potential application for in vitro constructing complex tissues and organs.
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