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3D ultramicroscopy (light sheet microscopy) is a versatile imaging technique with a unique combination of capabilities. It provides high imaging speed, high signal-to-noise ratio and low levels of photobleaching and phototoxic effects. These properties are critical in a wide range of applications in the life sciences. When combined with tissue clearing methods, light sheet microscopy allows rapid imaging of large specimens with excellent coverage and high spatial resolution. Even samples up to the size of entire mammalian brains can be efficiently recorded and quantitatively analyzed. Numerous examples of application are given.
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