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A new method for D2O/H2O exchange in infrared spectroscopy of proteins
Marco Malferrari, Giovanni Venturoli, Francesco Francia, Alberto Mezzetti
165 - 169
10.3233/978-1-61499-184-7-165
Advances in Biomedical Spectroscopy
Volume 7: Spectroscopy of Biological Molecules
Abstract

In this article, we describe a new method to obtain D2O/H2O exchange in photosynthetic reaction centres from Rhodobacter sphaeroides. The method is characterized by: (i) a very high efficiency of the isotopic replacement; (ii) an extremely low amount of D2O needed; (iii) the short time required for dehydration and D2O rehydration; (iv) the possibility of controlling concomitantly the hydration state of the sample. The proposed method can be applied to other proteins.

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