The ß-spectrin family of proteins was the subject of the analysis of amino acid replacenents at aligned positions. The homologous and non-homologous positions were subjected to an analysis of the interrelations among occurring residues and the mechanism of variability using the algorithm of genetic semihomology [6]. 67 ß-spectrin sequences were collected and 55 of them were subjected to an comparative analysis. After in-depth studies of the global multiple alingnment, a consensus sequence was construscted. It was the base of the detailed analysis of genetic relations among all the amino acid residues occuring the same positions of homologous sequences. Such examination shows a detailed picture of the relations among the representatives of the ß-spectrin family and gives a possibility of following the evolutionary paths of the protein family arising, what is the base of further analytic examinations of the ß-spectrin family.