Synchrotron light is million-times brighter than sunlight. Synchrotron-based infrared light is thousands times than globar-sourced infrared light. With synchrotron-radiation based infrared microspectroscopy (SR-IMS), a non-invasive and most powerful research tool has been developed, which can be used to study inherent structure and biopolymer conformations of biomaterials at cellular and molecular levels. The objective of this research program was to use synchrotron-based SR-IMS with molecular chemistry imaging technique plus multivariate spectral analyses to study various biopolymers distribution and intensity at four different locations in a modeled feed tissue (sorghum bicolor L.) within intact tissue. The biopolymers investigated are highly associated with feed and food nutrient availability and included both easily-digested and digestion-resistant biopolymers in complex plant seed systems. The experiment of imaging in complex tissue system was conducted at the U2B beamline station at National Synchrotron Light Sources, Brookhaven National Laboratory (U.S. Dept of Energy). The unique chemical functional groups which are associated with biopolymers were analyzed using molecular spectroscopic technique. The results showed that the architecture of the complex feed system and distribution of biopolymers intensity and their ratios within four different locations in the modeled seed tissue could be revealed at an ultra-spatial resolution within cellular dimensions. The multivariate molecular spectral analyses are conclusive in showing that they can discriminate and classify the different inherent structures within the seed tissues designed for feed and food purpose. Other applications and future direction in diagnosis and screening of feed and food quality are also discussed.