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Single cell studies for biomedical applications gain increased importance. Raman spectroscopy, especially when combined with optical tweezers, has proven to be a valuable tool for cancer cell screening, malaria detection, stem cell research as well as bacterial identification only to name a few applications. Here, basic principles behind methods, experimental design and parameters as well as expected results will be discussed. The goal is to give insight into experimental possibilities but also shortcomings that have to be dealt with regarding single cell Raman spectroscopy.
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