This chapter describes principles and applications of on-line coupling (hyphenation) of NMR spectroscopy with liquid-chromatographic and other separation methods as well as with other forms of spectroscopy. This results in highly integrated and automated systems for structure determination of components of complex mixtures at microgram and nanogram levels. Hyphenated NMR techniques provide accelerated and detailed insight into components of complex mixtures of biological origin without initial separation of the constituents.
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