The advent of isotope labeling strategies for macromolecules has enabled the application of multidimensional heteronuclear NMR experiments to biomolecular systems of unprecedented size and complexity. The improved signal dispersion afforded by multiple dimensions; however, comes at a price: each added dimension exponentially increases the required NMR data acquisition time if the conventional approach of Fourier Transform NMR is employed. Efforts to overcome this time barrier have been given additional impetus due to technological advances such as the advent of cryogenically cooled probes, increased strength of high-field magnets, and improvements in computation. In this chapter we review the most successful methods for accelerating NMR data acquisition, highlighting relationships among the methods as well as their respective strengths and weaknesses. Our aim is to provide a practical reference guide for researchers to better understand this rapidly growing field and help identify the approach best suited to a particular problem.