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The Study of Protein Reactions by Reaction-Induced Infrared Difference Spectroscopy
Andreas Barth
53 - 78
10.3233/978-1-60750-045-2-53
Advances in Biomedical Spectroscopy
Volume 2: Biological and Biomedical Infrared Spectroscopy
Abstract

Reaction-induced infrared difference spectroscopy of proteins is reviewed. This technique enables detailed characterization of enzyme function on the level of single bonds of proteins, cofactors or substrates. Discussed are methods to initiate protein reactions in the infrared samples, general aspects of spectra interpretation, measurements of enzyme activity and studies of protein function at the example of the Ca2+ pump.

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