Synchrotron Radiation Circular Dichroism (SRCD) spectroscopy is enabling a number of new applications in biology because of its ability to measure lower wavelength data, lower requirement for sample quantities, higher signal-to-noise levels and ability to examine proteins under a wide range of conditions. Advances in SRCD instrumentation, data collection methodologies, data processing, reference datasets and methods of analysis have lead to new applications of SRCD for examining interesting biological questions. Although most of the SRCD studies to date have focused on proteins, the technique has been successfully applied in the study of other macromolecules including DNA and carbohydrates. This chapter briefly discusses a number of examples of studies that have been undertaken with this new methodology.