The investigation of pedestrian spatio-temporal behaviour is of particular interest in many different research fields. Disciplines like travel behaviour research and tourism research, social sciences, artificial intelligence, geoinformation and many others have approached this subject from different perspectives. Depending on the particular research questions, various methods of data collection and analysis have been developed and applied in order to gain insight into specific aspects of human motion behaviour and the determinants influencing spatial activities. In this contribution, we provide a general overview about most commonly used methods for monitoring and analysing human spatio-temporal behaviour. After discussing frequently used empirical methods of data collection and emphasising related advantages and limitations, we present seven case studies concerning the collection and analysis of human motion behaviour following different purposes.