Bioinformatics and MD approaches are highly complementary tools for structural predictions. We have used these two approaches together to investigate structural and functional properties of tetrameric voltage-gated ion channels for which structural information is completely (CNG channels) or partially (HCN channels) lacking. Besides their predicting power, MD simulations have allowed to provide insights on functional properties of HCN channels. In addition, bioinformatics has allowed extending many of our findings obtained for a protein from one specific organism to the entire class of HCN and CNG channels, and MD to other proteins featuring the same fold but with totally different function.