The main attention is paid to some chemicals related to the group named as endocrine disrupting factors. First of all, it is observed the proposed methods based on the biosensorics principles and intended for the express estimation of total toxicity at the screening analysis of environmental objects, revealing of individual toxins and for the diagnostics of diseases induced by viruses. At the beginning the efficiency of the developed biosensor is analyzed in case of the work with the standard solution. Then the obtained results are compared with that received at the application of biosensors in real conditions. The working prototypes of biosensors are created for the application in the area of human and veterinary medicine, ecology and biotechnology.