This paper reports the results of development and test of Laser-Based Point Detector (LBPD) biodetector for on-line identification of biological warfare materials. The test results of data evaluation of its potential to identify four microorganisms (spores of Bacillus anthracis and Bacillus cereus, vegetative cells of Brucella abortus and Escherichia coli) in pure and mixed cultures at varying concentrations on-line are presented. The LBPD was tested using wireless communications against not only simulants but pathogens as well. Identification specific pathogenic organisms on-line, in a semi-automated process without reagents achieve promising results (87%).