Biological warfare agents are the most problematic weapons of mass destruction and terror. Both civilian and military sources predict that over the next decade the threat from proliferation of these agents will increase significantly. Therefore, the ability to accurately predict the dispersion, concentration, and ultimate fate of biological warfare agents released into the environment in real time is essential to prepare for and respond to a biological warfare agent release. A fusion of micro- and nanotechnologies with biosciences could significantly counter biological threat agents on the battlefield. Miniaturization of biosensor technologies has great potential for improving resolution time (speed of assay), reducing reagent use, and allowing for higher sample throughput. Fast analysis and on-chip integration of supporting electronic circuitry for signal analysis and remote control would enable sensing at a remote location. This paper describes a new biosensor technology based on combination of direct bioelectrocatalysis and multi-microchannel technology. To demonstrate direct electron transfer, glucose oxidase and PQQ-dependent glucose dehydrogenase have been selected. An electrochemical sensor, which includes biological sensing element immobilized on the surface of microchannels of a working electrode, can be used in the form of a flow-through amperometric, potentiometric, or conductometric device.