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The key research questions must be directed to the effects of UV on NO generation and action in plants. Research programmes will require methods to assess accurately NO emissions from leaves and to determine NO concentrations in cells and sub-cellular microdomains; the use of mutants and transgenic plants altered in NO synthetic and scavenging capacities; analyses of the molecular and biochemical events required for activation of PCD by NO and UV; and the development of techniques to monitor simultaneously cell death, NO and ROS generation in the field during exposure to UV.
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