This study examines mediated communication behavior in distributed networks of practice (DNoPs) in a multinational enterprise working in the marine insurance industry. The study describes and compares mediated communication behavior in five different distributed networks of practice as a combination of the knowledge activities that take place during communicative action, the media used to support communication, the networks' perceptions of different media, and the contextual factors that influence both communication and media selection. The networks experienced several challenges in the communication process such as technological instabilities that excluded participation, complex and highly equivocal messages, physical and social-psychological distance between participants, and media limitations. Different theoretical perspectives for mediated communication provide a framework for discussion and integration of the empirical findings in this study.