Nowadays, many hospitals have adopted information technology (IT) to help nurses in their practice; therefore, computers have become critical instruments for nurses. Many researchers have studied what information literacy or computer competencies a nurse should possess, but less research has focused on the types of factors associated with computer literacy. The purpose of this study was to discover which variables influence the computer literacy of nurses. The questionnaire was the instrument for data collection. The respondents were 180 nurses in Taiwan. The results of this study revealed that computer experience of nurses and personal innovation in IT were both factors associated with computer literacy and computer anxiety. The findings could assist nursing managers to screen computer-literate nurses and course designers in nursing school or hospitals to add more practical trainings in courses.