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In this work, the Design of Experiment (DOE) was applied to a Single Stage Multi Product Kanban System verification. The study was begun with the construction of a simulation model in Arena and continued with the optimization with OptQuest, in relation to the number of kanban cards in a kanban board placed between two stations of a production plant. Eventually, the model was then tested for system robustness verification, using DOE approach. Specifically, the aim of this work is to demonstrate the effectiveness of a methodology that, through the implementation of the DOE analysis, allows identifying those parameters that mostly influence the output of a simulation model previously implemented with ARENA.