The present article deals with the synthesis of amphiphilic compounds bearing single hydrophobic parts including hydrocarbon, perfluorocarbon and hydrofluorocarbon chains. From the physicochemical study of their behavior in an aqueous medium it appears that their organization depends mainly on the hydrophobic structure. Dynamically stable aggregates could be obtained with certain hybrid species whereas perfluorinated analogues would lead to micellar systems. Supramolecular organizations such as Newton Black Films (NBF) can be obtained with perfluorocarbon products, on the opposite the presence of an ethyl terminus part would prohibit the formation of films. Hybrid amphiphiles have been found to maintain membrane proteins in aqueous media. This class of compound could be used in the future to form supramolecular assemblies involving biomolecules.