Bio-printed cartilage with living cells is a regenerative cartilage scaffold that has living cells 3D printing out for the treatment of osteoarthritis and other conditions that damage articular cartilage. It is a new type of scaffold that is biocompatible, biomimetic, regenerative, and long term in use. Currently, there is a clinical and market lack of products for the regeneration of therapeutic cartilage, so the need for such scaffolds is necessary. Bio-printed cartilage with living cells from concept to product development and non-clinical and clinical testing to market launch requires evaluation of the scaffold for safety, efficacy, and compliance with specific requirements of the medical directives regulation and combined advanced therapy medicinal products.