We aimed to understand nursing informaticists’ perspectives on key challenges, questions, and opportunities for the nursing profession as it prepares for an era of healthcare delivery enriched by artificial intelligence (AI). We found that nursing practice is currently, and will continue to be, directly influenced by AI in healthcare. Educating and training nurses so that they may safely and effectively use AI in their clinical practice and engage in implementation planning and evaluation will help overcome predicted challenges. Defining the key tenets of AI literacy for nurses and re-envisioning nursing models of care in the context of AI-enriched healthcare are important next steps for nursing informaticists. If embraced, AI has the potential to support the existing nursing workforce in the context of major shortages and augment the safe and high-quality care that nurses can deliver.