In this work, an attempt has been made to analyze the influence of the frequencies bands in uterine electromyography (uEMG) signals on the detection of preterm birth. The signals recorded from the women’s abdomen during pregnancy are considered in this study. The signals are subjected to preprocessing using digital bandpass Butterworth filter and decomposed into different frequency bands namely, 0.3-1.0 Hz (F1), 1.0-2.0 Hz (F2) and 2.0-3.0Hz (F3). Spectral features namely, peak magnitude, peak frequency, mean frequency and median frequency are extracted from the power spectrum. Classification models namely, k-nearest neighbor, support vector machine and random forest are employed to distinguish the term and preterm conditions. The results show that the features extracted from these frequency bands are able to differentiate term and preterm condition. Particularly, the frequency band F3 performs better than other frequency bands. The features associated with these frequencies along with random forest classification model achieves a maximum accuracy of 75.2%. Thus, these measures could be used to accurately detect the preterm birth well in advance.