The crack of concrete will seriously affect the stability and durability of its structure. The microbial mineralization and crystallization technology can effectively improve the crack of concrete. In order to improve the influence of microbial self-healing technology on the mineralization and crystallization of concrete cracks, the South China Sea coral reef calcrete sand was used as a new porous material to support Pasteurella. The microbiological mineralized products were analyzed by SEM-EDS. The results showed that the microbiological mineralized products were mainly calcium carbonate crystals. The carbonization test was carried out to verify the repair effect of the resin on concrete cracks. The results showed that the content of bacteria carrier should not exceed 10%. When the bacterial carrier content is 10% and the mineralization reaction product content is 3%, the carbonization resistance of cement mortar specimen is improved most obviously. This research provides help for the engineering application of concrete crack repair and has high application value.