In this qualitative study, we explored the nurses’ experience in using hospital information systems (HIS) to identify barriers and facilitators of using this system. We interviewed twenty one purposefully-selected nurses who have experience in using HIS and analyzed the data using conventional content analysis. We identified 17 facilitators and 12 barriers classified into main themes. Lack of support for nurses, their resistance, any force for using HIS, perceived difficulty of using HIS, inadequate system quality, data loss, discontinuity of information in different systems are the main barriers. However, considering nurses’ perspectives, pilot implementation of HIS, training, planned implementation, appropriate financial and non-financial incentives, adding new and appropriate functionalities, increased ease of use and usability, easy access to information, improved data quality in HIS, and saving nurses’ working time through using HIS result in increased adoption the system to use.